Part:BBa_K2213004
PduD(1-20)_cgPPK2_mCherry_His6
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Usage and Biology
Fusion of the PduD(1-20), Corynebacterium glutamicum class II polyphosphate kinase and mCherry followed by a hexahistidine tag.
The construct was heterologously expressed in a BL21 (DE3) strain of E. coli under the control of a T7 promoter. The construct was cleaved before or during purification, resulting in the elution of a ~30 kDa (expected wieght: 66 kDa), his-tagged protein with a bright pink colour from the Ni-NTA agarose media (Figure 1). The protein fragment was not tested for kinase activity.
Figure 1. SDS-PAGE analysis of fractions from the Ni-NTA agarose purification of the PduD(1-20)_cgPPK_mCherry construct. The black arrow indicates where the expected band should be (66 kDa) in the elution fraction lane. Ladder: Precision Plus Protein™ (Biorad)
Sequence and Features
Illegal SapI.rc site found at 827
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